HsSAS-6-dependent cartwheel assembly ensures stabilization of centriole intermediates
نویسندگان
چکیده
منابع مشابه
Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells
SAS-6 proteins are thought to impart the ninefold symmetry of centrioles, but the mechanisms by which their assembly occurs within cells remain elusive. In this paper, we provide evidence that the N-terminal, coiled-coil, and C-terminal domains of HsSAS-6 are each required for procentriole formation in human cells. Moreover, the coiled coil is necessary and sufficient to mediate HsSAS-6 centros...
متن کاملCell-free reconstitution reveals centriole cartwheel assembly mechanisms
How cellular organelles assemble is a fundamental question in biology. The centriole organelle organizes around a nine-fold symmetrical cartwheel structure typically ∼100 nm high comprising a stack of rings that each accommodates nine homodimers of SAS-6 proteins. Whether nine-fold symmetrical ring-like assemblies of SAS-6 proteins harbour more peripheral cartwheel elements is unclear. Furtherm...
متن کاملDirect interaction of Plk4 with STIL ensures formation of a single procentriole per parental centriole
Formation of one procentriole next to each pre-existing centriole is essential for centrosome duplication, robust bipolar spindle assembly and maintenance of genome integrity. However, the mechanisms maintaining strict control over centriole copy number are incompletely understood. Here we show that Plk4 and STIL, the key regulators of centriole formation, form a protein complex that provides a...
متن کاملCartwheel assembly
The cartwheel is a subcentriolar structure consisting of a central hub and nine radially arranged spokes, located at the proximal end of the centriole. It appears at the initial stage of the centriole assembly process as the first ninefold symmetrical structure. The cartwheel was first described more than 50 years ago, but it is only recently that its pivotal role in establishing the ninefold s...
متن کاملSAS-6 is a Cartwheel Protein that Establishes the 9-Fold Symmetry of the Centriole
Centrioles consist of nine-triplet microtubules arranged in rotational symmetry. This structure is highly conserved among various eukaryotic organisms and serves as the base for the ciliary axoneme. Recently, several proteins such as SAS-6 have been identified as essential to the early process of centriole assembly, but the mechanism that produces the 9-fold symmetry is poorly understood. In C....
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ژورنال
عنوان ژورنال: Journal of Cell Science
سال: 2019
ISSN: 1477-9137,0021-9533
DOI: 10.1242/jcs.217521